Product Description:

This adipogenic differentiation medium kit is suitable for MSCs from different sources (e.g., ADSCs, UC-MSCs, BMSCs). Under induction, MSCs differentiate into mature adipocytes with lipid droplets, which are stained red by Oil Red O, indicating adipogenic differentiation. The degree of differentiation can be assessed by the area and intensity of red staining.

Product Information：

Product Advantages：

●High-efficiency adipogenic induction

●Suitable for MSCs from multiple sources

●Strict quality control with precise and reliable

Product Analysis:

▼Adipogenic differentiation result analysis

Under microscopic observation, large red-stained areas are visible. At 20X objective, red lipid droplets can be observed within cells, indicating the adipogenic capacity of MSCs. (The image shows induction results from cells that are less prone to adipogenesis. Version 2.0 can shorten the induction time to approximately 7–14 days.)

▼Adipogenic differentiation quantitative analysis

Semi-quantitative analysis of adipogenic staining using a microplate reader showed that this product achieved higher OD values than competitors, indicating superior adipogenic efficiency.

成脂诱导分化培养基使用说明书.pdf

Q: During induction, can gelatin coating solution be added to prevent cells from curling up and detaching? How exactly should this be done?

A: Under normal circumstances, this product does not require gelatin coating. If gelatin coating is needed, we offer a separate Gelatin Coating Solution (Catalog No. KC010643). To use it, add an appropriate amount of gelatin coating solution to cover the bottom of the plate, incubate in a laminar flow hood or cell culture incubator for 30 min, then aspirate the gelatin coating solution. The plate is then ready for cell seeding.

Q: Which surface markers are typically detected after induction?

A: Surface marker detection can refer to the standards established by the International Society for Cellular Therapy (ISCT) in 2006. Our routine procedure involves testing 10 markers to cover the commonly used indicators. These include negative markers: CD11b, CD31, CD34, CD45, HLA-DR; and positive markers: CD29, CD73, CD90, CD105, CD166. Special attention should be paid to the positivity of CD105.

Q: How many days are required for adipogenic induction?

A: Our internal testing results show that with this product, large-area, deeply stained lipid droplets can typically be observed under the microscope after 21 days of induction. Under 20× objective, numerous red lipid droplets can be seen within cells. Using the enhanced version, the induction time can be shortened to 7–14 days.