Product Description：

Bone Marrow Mesenchymal Cell Serum-Free Medium consists of a serum-free basal medium and a supplement. Mix to form a complete medium before use (antibiotic-free). Serum-free, xeno-free. Suitable for primary and subculture expansion of human and non-human primate bone marrow-derived mesenchymal stem cells and mesenchymal stromal cells.

Product Information：

Product Advantages：

Serum-free, xeno-free, compatible with phenol red (+/–)

Rapid cell proliferation, maintains stemness

Suitable for primary culture,no coating, low-density seeding

Suitable for large-scale production, supernatant collection

Low batch variation, IND-friendly

Broad applications

Product Analysis：

▼Microscopic observation of bone marrow mesenchymal stem cells：

Under an inverted microscope at 4X objective, bone marrow mesenchymal stem cells exhibit typical colony morphology. The cells are adherent, displaying a uniformly elongated spindle shape.

Under an inverted microscope at 10X objective, bone marrow mesenchymal stem cells are adherent and exhibit a uniformly elongated spindle-shaped morphology.

▼Flow cytometry immunophenotyping of bone marrow mesenchymal stem cells: positive markers > 95%, negative markers (sum) < 2%.

Cell surface markers：

CD29、CD44、CD73、CD90、CD105、CD14、CD19、CD34、CD45、HLA-DR

The positive rates are as follows：

100.00%、99.63%、99.99%、99.99%、99.99%、0.16%、0.01%、0.00%、0.06%、0.01%

骨髓间充质细胞无血清说明书.pdf

A: Yes. This medium supports primary culture, passaging, and proliferation of bone marrow-derived mesenchymal stem cells (MSCs) and mesenchymal stromal cells.

Q: Is there a phenol red-containing version of this medium?

A: Yes. This product is available in two versions: with phenol red and without phenol red.

Q: How should I switch from my existing culture system to this medium?

A: You may switch directly during passaging. An initial temporary reduction in cell proliferation capacity may occur during the adaptation period. The time to reach 80–90% confluence may increase by approximately one day. No special treatment is required; simply continue with routine passaging.

Q: Why does a white, hazy film appear during cell culture?

A: This occurs when cell density is too high and excessive extracellular matrix is produced by the cells, forming a white film. It is recommended to passage cells when confluence reaches 80–90%. Alternatively, you may extend the trypsin digestion time slightly, and stop the digestion once the cells have dispersed into single cells.