Product Description:

Canine Mesenchymal Cell Serum-Free Medium consists of Canine Mesenchymal Cell Serum-Free Basal Medium and Canine Mesenchymal Cell Medium Supplement, which must be mixed to form complete medium before use (antibiotic-free). This medium is suitable for the proliferation and culture of canine mesenchymal cells and mesenchymal stromal cells.

Canine Mesenchymal Cell Serum-Free Medium is a high-performance culture system specifically designed for the in vitro culture and expansion of canine-derived mesenchymal stromal cells and mesenchymal cells with stem-like characteristics. This medium requires no supplementation with traditional animal serum (such as fetal bovine serum). Through an optimized nutrient composition and a precisely controlled combination of growth factors, it provides a stable and supportive growth microenvironment for cells, effectively promotes proliferation, maintains the typical phenotype, and inhibits spontaneous differentiation. Its formulation significantly enhances the proliferation efficiency of canine mesenchymal cells while preserving the stability of their morphology, function, and phenotype, making it suitable for various basic research and preclinical research needs.

Product Information：

※Suitable for primary and subculture of canine mesenchymal stem cells and mesenchymal stromal cells. (Phenol red-free version available.)

Product Analysis:

▼Tri-lineage differentiation

Under microscopic observation, large stained areas indicate the tri-lineage differentiation capacity of canine mesenchymal stem cells.

▼Cell surface markersCD29、CD44、CD34、HLA-DR

   The positive rates are as follows：99.95%、99.99%、0.22%、0.1%

犬间充质细胞无血清说明书.pdf

Q: Does this medium support primary cell isolation?
A: Yes, this medium supports the primary and subculture proliferation of canine-derived mesenchymal stem cells and mesenchymal stromal cells. It is available in two versions: with phenol red and without phenol red.

Q: How should the medium be prepared into complete medium before use?
A: Add the Canine Mesenchymal Cell Medium Supplement to the Canine Mesenchymal Cell Serum-Free Basal Medium at a volume ratio of 1:25. For example, add 20 mL of the supplement to 500 mL of the basal medium and mix thoroughly to obtain complete medium.

Q: How should the medium be thawed and rewarmed before use?
A: The basal medium is stored at 2–8°C. Before use, remove it from the refrigerator and allow it to rewarm at room temperature.
The supplement is stored at –20°C. Before use, thaw it completely at 4°C or at room temperature.

Q: How long can the prepared complete medium be stored?
A: The prepared complete medium can be stored at 2–8°C protected from light for up to 4 weeks.