Product Description:

This chondrogenic differentiation medium kit is suitable for MSCs from different sources (e.g., ADSCs, UC-MSCs, BMSCs). Under induction, MSCs differentiate into chondrocytes. Alcian Blue staining shows acidic mucopolysaccharides in cartilage, indicating chondrogenic differentiation.

Product Information：

Product Advantages：

• High-efficiency chondrogenic induction

• Suitable for MSCs from multiple sources

• Strict quality control with precise and reliable

Product Analysis:

▼Chondrogenic differentiation (3D)

Large blue-stained areas are observed under the microscope, demonstrating the chondrogenic capacity of MSCs via Alcian Blue staining.

▼Chondrogenic differentiation (2D)

Large blue-stained areas (Alcian Blue positive for acidic mucopolysaccharides) are observed under the microscope, demonstrating the chondrogenic capacity of MSCs.

成软骨诱导分化培养基使用说明书.pdf

Q: After differentiation using the chondrogenic induction medium, is the resulting cartilage hyaline cartilage or fibrocartilage?
A: Theoretically, it is fibrocartilage.

Q: Can the gelatin coating solution provided in the differentiation induction kit be recycled and reused?
A: Generally, recycling is not recommended. However, if the customer has a limited amount of coating solution, it can be reused, but no more than three times. Aseptic technique must be maintained during the process. We also sell gelatin solution separately (Catalog No. KC010643), which can be purchased individually.

Q: Is sealing (coverslipping) required after chondrogenic induction staining?
A: After the chondrogenic induction and result detection, Alcian Blue is used to stain the sections, and the stained results are recorded by photography. At this point, the cells are already dead and are generally not used for any other purposes. Therefore, coverslipping is not required.

Q: How many days are needed for chondrogenic induction?
A: Our internal test results show that with this product, relatively large and deeply stained spots can typically be observed under the microscope after 15 days of induction.