Product Description：

A highly efficient and gentle cell dissociation enzyme for adherent cell passaging, primary cell isolation, and tissue/organoid dissociation. Compared to traditional trypsin, it better protects cell viability and ensures reproducibility, ideal for trypsin-sensitive cells. Made from recombinant proteins, animal-free, safe, with minimal batch-to-batch variation.

Product Information：

Product Advantages：

• Animal  component-free

• High  stability

• Ready-to-use liquid

• Gentle and efficient

• Wide application range

• Meets diverse scenario needs

Product Analysis:

Mesenchymal stem cells are one of the cell types more sensitive to digestive enzymes. Umbilical cord MSCs were digested by immersion in this product, and viability tests were performed at different time points, as shown in the table below：

After up to 60 minutes of immersion digestion challenge, cell viability remained as high as 94%, demonstrating that this product maintains cell activity well with minimal cell damage.

This product (digestion solution) was compared with similar imported products to evaluate the performance of umbilical cord mesenchymal stem cells in the following aspects:

Comparative tests showed no differences in cell morphology, digestion time, viability, or yield between this product and similar imported products. This product delivers equivalent digestion performance with the same stability and reliability.

细胞消化液说明书.pdf

Q: What are the main components of this dissociation solution?
A: It is mainly composed of recombinant trypsin, EDTA, and DPBS. It contains no animal-derived components, no phenol red, and no antibiotics.

Q: How long should the digestion time be when using this dissociation solution?
A: Once the cells shrink, become round, and detach from the bottom of the culture flask, add 5 mL of complete medium to terminate the digestion.

Q: Does using this dissociation solution damage cells?
A: No, this product causes very little damage to cells. Tests have shown that after 60 minutes of exposure to this dissociation solution, cell viability remains as high as 94%.